The Frankford and Southwark Passenger Railway Company was the first street railway company to be
incorporrated in Philadelphia and was chartered April 4, 1854. The second company to be chartered
was the
Philadelphia and Darby Railway Company chartered on April 28, 1857 . (Source  
"Fifty Years with Passenger Railways of Philadelphia -Semi-Centennial Number) The West
Philadelphia-Darby Railway was the
first to be integrated-
The line to Darby began operating 24 December, 1858
In 1884, Rufus C. Hartranft compiled a "Guide to the City Of Philadelphia" for the use of
members attending that year's  meeting of the American Association for the Advancement of
Science. Under
Phila. Pass. Railway Companies Specified Routes it says "Darby Road
Branch (blue cars)- from depot, forty-ninth and Woodland av. via Woodland av. to Chestnut to
Walnut to Twenty-Second, to Chestnut, to Woodland av, to depot. Exchanges with all cross
roads. Red cars from Darby via Woodland av., to Chestnut, to Thirty-second, returning via the
same route. Single fare, six cents, to Forty-ninth st; to Darby or west of Forty-ninth st., twelve
cents."   
PDF File of West Philadelphia-Darby Railway fares from July
1859 - From the Collection of Ed Springer