The Frankford and Southwark Passenger Railway Company was the first street railway company to be incorporrated in Philadelphia and was chartered April 4, 1854. The second company to be chartered was the Philadelphia and Darby Railway Company chartered on April 28, 1857 . (Source  "Fifty Years with Passenger Railways of Philadelphia -Semi-Centennial Number) The West Philadelphia-Darby Railway was the first to be integrated
The line to Darby began operating 24 December, 1858
PDF File of West Philadelphia-Darby Railway fares from July 1859 - From the Collection of Ed Springer
In 1884, Rufus C. Hartranft compiled a "Guide to the City Of Philadelphia" for the use of members attending that year's  meeting of the American Association for the Advancement of Science. Under Phila. Pass. Railway Companies Specified Routes it says "Darby Road Branch (blue cars)- from depot, forty-ninth and Woodland av. via Woodland av. to Chestnut to Walnut to Twenty-Second, to Chestnut, to Woodland av, to depot. Exchanges with all cross roads. Red cars from Darby via Woodland av., to Chestnut, to Thirty-second, returning via the same route. Single fare, six cents, to Forty-ninth st; to Darby or west of Forty-ninth st., twelve cents."